SKU: 30973258600

Rat TSH ELISA Kit

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Description

Rat TSH ELISA KitProduct Specification Usage Sample Processing and Requirements 1. Serum: Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4C overnight, then centrifuge at 1000g for 20 minutes. Remove the supernatant and store it at 20C or 80C. However, repeated freezing and thawing should be avoided. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Within 30 minutes of collection,

Product Specification

Usage

Sample Processing and Requirements

1.
Serum: Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.
Remove the supernatant and store it at -20°C or -80°C.
However, repeated freezing and thawing should be avoided.

2.
Plasma: Collect the specimen using EDTA or heparin as an anticoagulant.
Within 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.
Remove the supernatant and test it.
Alternatively, store it at -20°C or -80°C.
However, repeated freezing and thawing should be avoided.

3.
Tissue Homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).
Weigh the tissue and mince it.
Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.
The specific volume can be adjusted according to experimental needs and recorded.
It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.
To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.
Finally, centrifuge the homogenate at 5000×g for 5-10 minutes.
Remove the supernatant for analysis.

4.
Cell culture supernatant or other biological specimens: Centrifuge at 1000×g for 20 minutes.
Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.
Note: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.

Reagent Preparation
After removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.
Dilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.

Procedure
1.
Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.
Seal the remaining strips in a ziplock bag and return them to 4°C.

2.
Set up standard wells and sample wells.
Add 50 μL of standard solution of varying concentrations to each standard well.

3.
Add 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.

4.
Add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.
Seal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.

5.
Discard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).
Let stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.
Repeat this process five times (a plate washer can also be used).

6.
Add 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.

7.
Add 50 μL of stop solution to each well.
Within 15 minutes, measure the OD value of each well at a wavelength of 450 nm.

Calculation of Experimental Results:
Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.
Obtain a linear regression equation.
Substitute the OD value of the sample into the equation to calculate the sample concentration.
This figure is for reference only: Standard Curve.

Sensitivity The minimum detection concentration is less than 0.1 mU/L.
Species Reactivity Rat
Theory The kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are added sequentially to microwells pre-coated with a rat thyroid-stimulating hormone (TSH) capture antibody. The assay is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of rat thyroid-stimulating hormone (TSH) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.
Detection Type Used for in vitro quantitative detection of rat thyroid-stimulating hormone (TSH) content in serum, plasma, tissue homogenate and related liquid samples.
Composition

Name

96 T

Remarks

Microwell enzyme plate

12 holes×8 strips

None

Standard product

0.3mL*6 tube

None

Sample diluent

6mL

None

Detection antibody-HRP

10mL

None

20×Wash Buffer

25mL

Dilute according to instructions

Substrate A

6mL

None

Substrate B

6mL

None

Stop solution

6mL

None

Sealing film

2 Zhang

None

Remarks:

1. The concentrations of the standard substances are: 20, 10, 5, 2.5, 1.25, and 0.625 mU/L.

2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.

General Notes

1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.

2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.

3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.

4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.

5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.

6. Avoid direct exposure to strong light during storage and incubation.

7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.

8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.

Any bleaching component will destroy the biological activity of the reagents in the kit.

9. Do not use expired products.

10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures.

Storage Temp. Unopened test kit, stored at 2-8°C, has a shelf life of 6 months.
Test Range 0.625 mU/L – 20 mU/L.
Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
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SKU: 30973258600

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ARS
San Leandro, US
★★★★★ 5
Quality & Comfort with Fully Adjustable Support
Team Name: Eden, Size: Queen, Color: Classic
I used to buy pricey, handmade, 100% down pillows—until I learned about the cruelty behind down production. I stopped using down entirely: pillows, comforters, jackets, everything. Then began the long search for something that could at least come close to the comfort of down, I tried Tempur and countless others, but nothing came close. As a side sleeper, I need a pillow that’s high, SOFT, supportive, conforming, and huggable. After wasting money on at least five different pillows, I finally tried the Coop Home Goods Eden Pillow because of its adjustability. The cover is kind of thick, soft, and well‑made (I use a nice cotton pillow cover over it). The filling is soft and high quality. You can customize the height and firmness however you like. It even comes with plenty of extra fill. To my surprise, the shredded foam doesn’t make the pillow clumpy—something every other similar pillow I tried struggled with. The surface stays smooth and soft, and it’s been a total game‑changer. It’s a keeper! I’ve had mine for almost four years now and only needed to add more filling once, about 8–10 months ago. Toss it in the dryer on low with a couple of tennis balls, and it fluffs up like new. And the icing on the cake: outstanding customer service. I highly recommend this pillow and will definitely buy it again
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Reviewed in the United States on December 26, 2025
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Verified Purchase
Andrew L. Carpenter
Pawtucket, US
★★★★★ 3
Nothing like the original Eden, don’t buy the name
Team Name: Eden Cool+, Size: King, Color: Classic
I’ve had the original Coop Eden for 5+ years and it has been a great pillow, very comfortable, soft but supportive but when I purchased a new mattress I wanted to refresh my pillow and saw the Eden Cool+ and wanted to give it a try. I figured it could only be better than the original Eden…but it is not. While the outer cover is noticeably cooler to the touch, it is not soft like the Eden. Also, this cool material mostly gets negated if you use a pillow case anyway. The shaped fill material I would also say is “lumpier” for lack of a better word than the shredded material in the original. The combination of the harder, less supple outer casing and the lumpy material just aren’t a good combination. I will say it does sleep slightly cooler than the original Eden but not enough for the sacrifice in comfort. I would absolutely consider going back to the original Eden.
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Reviewed in the United States on February 26, 2026
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Verified Purchase
Chris B
Boise, US
★★★★★ 5
Unmatched Pillow with Unmatched Customer Service!!! 5 STAR ALL AROUND
Team Name: Eden Cool+, Size: King, Color: Classic
## A Dream Come True (and Customer Service to Match!) From the moment I first laid my head on the Coop Eden Cool+ pillow, I knew I'd found something special. It's truly a game-changer for hot sleepers like me. The "Cool+" technology isn't just a gimmick; it actually works to keep my head and neck comfortably cool throughout the night, leading to the most refreshing sleep I've had in years. The adjustable fill is brilliant, allowing me to customize the loft perfectly for my sleep style, which means no more waking up with neck pain. The support is fantastic – it cradles my head without feeling too firm, and it holds its shape beautifully. However, what truly elevates my experience to a full five stars, beyond the exceptional product itself, is the **dedicated and outstanding customer service**. I had a minor issue with my initial pillow (a rare manufacturing defect, which can happen with any product). I reached out to Coop Home Goods, and their response was nothing short of phenomenal. They were incredibly prompt, empathetic, and went above and beyond to resolve the issue swiftly and completely. They genuinely cared about my satisfaction and ensured I was left with a perfect product. It's rare to find a company that not only produces a superior product but also stands behind it with such integrity and genuine concern for their customers. The Coop Eden Cool+ pillow is an investment in your sleep, and knowing that you're backed by such an incredible customer service team makes it an even easier decision. Highly, highly recommend!
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Reviewed in the United States on August 8, 2025
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Amazon Customer
Fort Morgan, US
★★★★★ 5
Great for side sleepers
It always seems hard to find the perfect pillow when you’re a side-sleeper. This one is great. It comes compressed so you will want to allow some time for it to fully fluff back up. The cover is soft and smooth. I love that it isn’t overstuffed. It provides gentle support and a lot of comfort. I was worried it may be too warm since it is polyester, but it is very breathable and I have not had any issues with feeling overly warm on it. It fits a standard size pillow case. It’s not overly stuffed like some other pillows I’ve tried that were specifically for side-sleepers. I think this world be a great pillow for any sleeping position, really. I wasn’t expecting to love a pillow this affordable, but it gets the job done and I get a good night of sleep. I’ll be purchasing some more for our guest room.
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Reviewed in the United States on May 10, 2025
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Jimmylee
Port Orchard, US
★★★★★ 5
Incredibly Comfortable and Supportive — My New Favorite Pillow!
I wasn’t expecting to love this pillow as much as I do, but the DIORIS large sleeping pillow really exceeded my expectations. From the first night, I noticed a difference in the way my neck and shoulders felt in the morning—less tension, better alignment, and overall more restful sleep. The fill is just the right balance between plush and supportive. It doesn’t flatten out during the night, which is a problem I’ve had with other pillows, especially those that come overstuffed or too soft. This one holds its shape well but still has that cozy, cloud-like feel when you lay your head on it. I’m a side and back sleeper, and this pillow works beautifully in both positions. It’s also large enough that I don’t feel like I’m falling off it during the night, but not so big that it takes over the whole bed. One suggestion: if you’re picky about loft, you might want to give it a gentle fluff or adjust the fill before your first night. But honestly, I didn’t need to do much—it was pretty much perfect out of the bag. Highly recommended if you're looking for a high-quality, comfortable, and generously sized pillow that supports a great night’s sleep!
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Reviewed in the United States on May 9, 2025

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