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Description
Mouse CRT ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a calreticulin (CRT) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of calreticulin (CRT) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Calreticulin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Calreticulin (CRT), also known as calregulin, CRP55, CaBP3, calsequestrin-like protein, and endoplasmic reticulum-resident protein 60 (ERp60), is a protein encoded by the CALR gene. Calreticulin is a multifunctional soluble protein that binds to Ca2+ ions, inactivating them. It binds with low affinity but has a high capacity, allowing for release in response to signals. Calreticulin is located in storage compartments associated with the endoplasmic reticulum and is considered an ER-resident protein. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 196 reviews
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Product Reviews
★★★★★ 5
THE BEST LOTION I HAVE EVER USED
Scent: Sweet Vanilla & Soft Wood, Size: 18.5 Fl Oz (Pack of 1)
THIS WINTER AND HAVING THE HEAT ON HIGHER THEN I NORMALLY DO HAS DONE A NUMBER ON MY SKIN. IT IS SO DRY AND I HAVE TRIED SO MANY THINGS AND NOTHING HAS REALLY WORKED. SO I GAVE IN AND SPENT THE MONEY AND TRIED THIS. I AM SO GLAD I DID. THIS WAS WORTH EVERY PENNY. I LOVED IT SO MUCH I BOUGHT ANOTHER BOTTLE JUST TO HAVE SO I WOULDN'T RUN OUT. I START OUT WITH THE BODY WASH WITH THE OLAY SUPER SERUM BODY WASH AND JUST AFTER ONE WASH MY SKIN WAS MUCH SOFTER AND SMOOTHER. YOU DO NOT NEED A LOT AS IT LATHERS UP NICELY IT SMELLS GREAT AND IT RINSES OFF GREAT LEAVING YOUR SKIN FEELING CLEAN AND SOFT. IT DOESN'T HAVE A STRONG SCENT TO IT WHICH IS FINE . I FOLLOW IT WITH IT WITH THIS SUPER SERUM LOTION JUST TO MAKE SURE THE SKIN STAYS SOFT. THIS LOTION IS SO SOFT AND SILKY. IT ABSORBS QUICKLY INTO YOUR SKIN WITHOUT LEAVING YOUR SKIN FEELING GREASY. IT IS LIGHTWEIGHT AND LEAVES YOU FEELING SOFT WITHOUT THAT DRY CRACKED APPEARANCE. THE SCENT ISN'T VERY STRONG TO ME WHICH IS FINE BECAUSE I WEAR PERFUME SO I DO NOT HAVE TO WORRY ABOUT THE SCENTS MIXING. I DEFINITELY RECOMMEND THIS REGIMEN TO ANYONE SUFFERING FROM DRY SKIN.
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Reviewed in the United States on March 27, 2026
★★★★★ 4
4.5/5; Great product, but smells strong
Scent: Sweet Vanilla & Soft Wood, Size: 10 Fl Oz (Pack of 1)
Context:
To start off, I should say that I have dry skin. Like, VERY dry skin. In purchasing this item, I wanted a product that I could consistently apply every single day to hydrate my skin.
Product:
The application of this stuff was like a dream. If you’ve ever tried using ANY sort of lotion (Lubriderm, Cetaphil, etc.) you’d know that usually it leaves that greasy texture on your hands that you have to wash off. This one doesn’t do ANY of that. The cream melts into your skin and absorbs very easily, unlike other lotions. I didn’t need that much to apply to my entire body. The results were great—my skin looked better and felt softer. If there was to be a con, it would be how strong the fragrance of the lotion is. This stuff smells STRONG. It’s not an unpleasant scent, but if you wanted to wear a perfume or body spray along with it, good luck because I can’t see a world where the cream won’t overpower or at least show through your preferred scent.
TLDR
Overall, good product with dreamlike application, but strength of scent could be dealbreaker for some.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
This everyday body lotion is the bomb!
Scent: Collagen, Size: 17 Fl Oz (Pack of 1)
This body lotion is absolutely fabulous! It really does make the skin on my thighs and abdomen look and feel firmer and the skin on my arms, legs, hands, and feet is noticeably softer and more hydrated without looking greasy or feeling sticky. I even use it on my neck and my face, which has tended to be more dry than oily as I've aged. I also really appreciate that the scent is pleasant enough to stand on its own but subtle enough to work well with other fragrances. I don't think that I have ever been this satisfied with a health/beauty product at such an affordable price point. I even purchased a few extra bottles to gift to friends and family.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Wow - my new favorites 💜👍😀
Scent: Retinol, Size: 17 Fl Oz (Pack of 1)
I just purchased this and tried it last night for the first time along with the firming version. I didn't take photos because I was skeptical as a product I had purchased from a different line for more money didn't work. This morning I notice that not only does my skin feel more hydrated but the texture appears more smooth. I recently turned 75 & lost my husband a few months ago and having been a care giver for awhile and put taking care of me aside. These products and body wash are great and I like the fragrance.
Please give them a try. To have something show this much difference over night is incredible. They are also affordable which is a plus for me. Thank you Olay.
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Reviewed in the United States on April 24, 2026
★★★★★ 5
Wonderful
Scent: Sweet Vanilla & Soft Wood, Size: 18.5 Fl Oz (Pack of 1)
I've only tried it for the first time, so I can't speak to performance, but I love 5his Product! It isn't greasy, it absorbs quickly & my skin feels much softer. The scent is just beautiful. It's light & not closing like some. Recommend highly.
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Reviewed in the United States on May 18, 2026